64Cu-ATSM Hypoxia Positron Emission Tomography for Detection of ConduitIschemia in an Experimental Rat Esophagectomy Model

Collection with item attached
2015
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/473452.do
DOI
10.1371/journal.pone.0131083
Title
64Cu-ATSM Hypoxia Positron Emission Tomography for Detection of ConduitIschemia in an Experimental Rat Esophagectomy Model
Description
This study was supported by the National Research Foundation of Koreagrant funded by the Korean Government (no. 2012027176) and the NationalR&D Program for Cancer Control, Ministry of Health and Welfare(1320210).
abstract
Background
We designed a hypoxia-imaging modality to detect ischemia of the gastric conduit after esophagectomy.
Materials and Methods
A rat esophagectomy model was created using 12-16-week-old, 300-350 g male Sprague-Dawley rats. In the operation group (n=6), partial gastric devascularization was performed by ligating the left gastric artery and the short gastric arteries and an esophagogastric anastomosis was performed. In the control group (n=6), the esophageal-gastric junction was incised and suturing was performed without gastric devascularization. Positron emission tomography (PET) images were taken using a microPET rodent model scanner, 24 h after the initial operation, after injection of 200 mu Ci 64Cu-diacetyl-bis (N4-methylsemicarbazone) (64Cu-ATSM) and pimonidazole 120 mg/kg. After microPET imaging, autoradiography and immunohistochemistry were performed.
Results
The PET image revealed 64Cu-ATSM uptake at the fundus in the operation group 3 h after 64Cu-ATSM injection. The maximum percentage of the injected dose per gram of tissue was higher in the operation group (0.047 +/- 0.015 vs. 0.026 +/- 0.006, p=0.021). The fundus/liver ratio was also higher in the operation group (0.541 +/- 0.126 vs. 0.278 +/- 0.049, p=0.002). Upon autoradiography, 64Cu-ATSM uptake was observed in the fundus in the operation group, and was well-correlated to that observed on the PET image. Upon immunohistochemistry, expression of hypoxia-inducible factor 1a and pimonidazole were significantly increased at the fundus and lesser curvature compared to the greater curvature in the operation group.
Conclusion
Hypoxia PET imaging with 64Cu-ATSM can detect ischemia in a rat esophagectomy model. Further clinical studies are needed to verify whether hypoxia imaging may be useful in humans.
provenance
Made available in Cube on 2018-09-28T10:19:57Z (GMT). No. of bitstreams: 0
language
English
author
Park, Seong Yong
Kang, Won Jun
Cho, Arthur
Chae, Ju Ri
Cho, Ye Lim
Kim, Jung Young
Lee, Ji Woong
Chung, Kyung Young
accessioned
2018-09-28T10:19:57Z
available
2018-09-28T10:19:57Z
issued
2015
citation
PLOS ONE(10): 6
issn
1932-6203
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/473452.do
Funder
보건복지부
Funding Program
암연구소및국가암관리사업본부운영
Project ID
1465015333
Jurisdiction
Rep.of Korea
Project Name
Developement of cancer targeting theragnosis probe by radiolabeling of aptamer
rights
openAccess
type
article


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