3D-Printed Microfluidic Device for the Detection of Pathogenic BacteriaUsing Size-based Separation in Helical Channel with TrapezoidCross-Section

Collection with item attached
2015
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/473436.do
DOI
10.1038/srep07717
Title
3D-Printed Microfluidic Device for the Detection of Pathogenic BacteriaUsing Size-based Separation in Helical Channel with TrapezoidCross-Section
Description
This work was supported by the National Research Foundation of Korea(NRF) grant funded by the Korea government (MEST) (No.2014R1A2A2A01007027). W.L. acknowledges the grant from POSTECHPresidential Fellowship.
abstract
A facile method has been developed to detect pathogenic bacteria using magnetic nanoparticle clusters (MNCs) and a 3D-printed helical microchannel. Antibody-functionalized MNCs were used to capture E. coli (EC) bacteria in milk, and the free MNCs and MNC-EC complexes were separated from the milk using a permanent magnet. The free MNCs and MNC-EC complexes were dispersed in a buffer solution, then the solution was injected into a helical microchannel device with or without a sheath flow. The MNC-EC complexes were separated from the free MNCs via the Dean drag force and lift force, and the separation was facilitated in the presence of a sheath flow. The concentration of the E. coli bacteria was determined using a light absorption spectrometer, and the limit of detection was found to be 10 cfu/mL in buffer solution and 100 cfu/mL in milk.
provenance
Made available in Cube on 2018-09-28T10:19:30Z (GMT). No. of bitstreams: 0
language
English
author
Lee, Wonjae
Kwon, Donghoon
Choi, Woong
Jung, Gyoo Yeol
Jeon, Sangmin
accessioned
2018-09-28T10:19:30Z
available
2018-09-28T10:19:30Z
issued
2015
citation
SCIENTIFIC REPORTS(5)
issn
2045-2322
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/473436.do
Funder
교육부
Funding Program
BK21플러스사업
Project ID
1345244318
Jurisdiction
Rep.of Korea
Project Name
program for advanced education of creative Chemical Engineers
rights
openAccess
type
article


Files in This Item

There are no attached files.