3 '-UTR engineering to improve soluble expression and fine-tuning ofactivity of cascade enzymes in Escherichia coli

Collection with item attached
2016
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/473418.do
DOI
10.1038/srep29406
Title
3 '-UTR engineering to improve soluble expression and fine-tuning ofactivity of cascade enzymes in Escherichia coli
Description
This study was supported by the Marine Biomaterials Research Centergrant from the Marine Biotechnology Program funded by the Ministry ofOceans and Fisheries, Republic of Korea
abstract
3'-Untranslated region (3'UTR) engineering was investigated to improve solubility of heterologous proteins (e.g., Baeyer-Villiger monooxygenases (BVMOs)) in Escherichia coli. Insertion of gene fragments containing putative RNase E recognition sites into the 3'UTR of the BVMO genes led to the reduction of mRNA levels in E. coli. Importantly, the amounts of soluble BVMOs were remarkably enhanced resulting in a proportional increase of in vivo catalytic activities. Notably, this increase in biocatalytic activity correlated to the number of putative RNase E endonucleolytic cleavage sites in the 3'UTR. For instance, the biotransformation activity of the BVMO BmoF1 (from Pseudomonas fluorescens DSM50106) in E. coli was linear to the number of RNase E cleavage sites in the 3'UTR. In summary, 3'UTR engineering can be used to improve the soluble expression of heterologous enzymes, thereby fine-tuning the enzyme activity in microbial cells.
provenance
Made available in Cube on 2018-09-28T10:18:59Z (GMT). No. of bitstreams: 0
language
English
author
Song, Ji-Won
Woo, Ji-Min
Jung, Gyoo Yeol
Bornscheuer, Uwe T.
Park, Jin-Byung
accessioned
2018-09-28T10:18:59Z
available
2018-09-28T10:18:59Z
issued
2016
citation
SCIENTIFIC REPORTS(6)
issn
2045-2322
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/473418.do
Funder
교육부
Funding Program
BK21플러스사업(0.5)
Project ID
1345258412
Jurisdiction
Rep.of Korea
Project Name
Research team for convergence technology to develop value-added food products
rights
openAccess
type
article


Files in This Item

There are no attached files.