2,3-Dimethoxy-2 '-hydroxychalcone ameliorates TNF-alpha-induced ICAM-1expression and subsequent monocyte adhesiveness via NF-kappaB inhibitionand HO-1 induction in HaCaT cells

Collection with item attached
2016
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/473410.do
DOI
10.5483/BMBRep.2016.49.1.141
Title
2,3-Dimethoxy-2 '-hydroxychalcone ameliorates TNF-alpha-induced ICAM-1expression and subsequent monocyte adhesiveness via NF-kappaB inhibitionand HO-1 induction in HaCaT cells
Description
This work was supported by the Regional Research UniversitiesProgram/Medical & Bio-material Research Center Grant, by the PriorityResearch Centers Program Grant (2009-0093812). This work was alsosupported by a Grant (HRF-G-2015-2) from Hallym University.
abstract
Up-regulation of adhesion molecules plays an important role in the infiltration of leukocytes into the skin during the development of various inflammatory skin diseases, such as atopic dermatitis. In this study, we investigated the modulatory effects of 2,3-dimethoxy-2'-hydroxychalcone (DMHC) on tumor necrosis factor (TNF)-alpha-induced intercellular adhesion molecule-1 (ICAM-1) expression and monocyte adhesiveness, as well as the molecular mechanisms underlying its action in the HaCaT human keratinocyte cell line. Pre-treating HaCaT cells with DMHC significantly suppressed TNF-alpha-induced ICAM-1 expression and subsequent monocyte adhesiveness. DMHC inhibited TNF-alpha-induced activation of NF-kappa B. In addition, DMHC induced HO-1 expression as well as NRF2 activation. Furthermore, HO-1 knockdown using siRNA reversed the inhibitory effect of DMHC on TNF-alpha-induced ICAM-1 expression and adhesion of monocytes to keratinocytes. These results suggest that DMHC may inhibit TNF-alpha-induced ICAM-1 expression and adhesion of monocytes to keratinocytes by suppressing the signaling cascades leading to NF-kappa B activation and inducing HO-1 expression in keratinocytes.
provenance
Made available in Cube on 2018-09-28T10:18:46Z (GMT). No. of bitstreams: 0
language
English
author
Kim, Hyejin
Youn, Gi Soo
An, Soo Yeon
Kwon, Hyeok Yil
Choi, Soo Young
Park, Jinseu
accessioned
2018-09-28T10:18:46Z
available
2018-09-28T10:18:46Z
issued
2016
citation
BMB REPORTS(49): 1
issn
1976-6696
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/473410.do
Funder
교육부
Funding Program
이공학학술연구기반구축
Project ID
1345250342
Jurisdiction
Rep.of Korea
Project Name
Development of Therapeutic Drugs by using the Biomolecule Delivery System; Screening and Development of Novel Macromolecules for Clinical Therapy of Human Diseases
rights
openAccess
subject
Adhesion
HO-1
Keratinocyte
NF-kappa B
TNF-alpha
type
article


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