14-3-3 proteins regulate Tctp-Rheb interaction for organ growth inDrosophila

Collection with item attached
2016
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/473403.do
DOI
10.1038/ncomms11501
Title
14-3-3 proteins regulate Tctp-Rheb interaction for organ growth inDrosophila
Description
We are grateful to Drs Kyung-Ok Cho and Efthimios Skoulakis for criticalcomments on the manuscript, and to Dr Nahum Sonenberg for generoussharing of anti-Thor antibody. We acknowledge the Bloomington DrosophilaStock Center, the National Institute of Genetics stock center, theVienna Drosophila Resource Center, the Drosophila Genomics ResourceCenter and the Developmental Studies Hybridoma Bank for fly stocks andantibodies. This research was supported by a National ResearchLaboratory grant (NRF-2011-0028326) and a Global Research Laboratorygrant (2014K1A1A2042982) through the National Research Foundation ofKorea, funded by the Korean, Ministry of Education, Science &Technology.
abstract
14-3-3 family proteins regulate multiple signalling pathways. Understanding biological functions of 14-3-3 proteins has been limited by the functional redundancy of conserved isotypes. Here we provide evidence that 14-3-3 proteins regulate two interacting components of Tor signalling in Drosophila, translationally controlled tumour protein (Tctp) and Rheb GTPase. Single knockdown of 14-3-3 epsilon or 14-3-3 zeta isoform does not show obvious defects in organ development but causes synergistic genetic interaction with Tctp and Rheb to impair tissue growth. 14-3-3 proteins physically interact with Tctp and Rheb. Knockdown of both 14-3-3 isoforms abolishes the binding between Tctp and Rheb, disrupting organ development. Depletion of 14-3-3s also reduces the level of phosphorylated S6 kinase, phosphorylated Thor/4E-BP and cyclin E (CycE). Growth defects from knockdown of 14-3-3 and Tctp are suppressed by CycE overexpression. This study suggests a novel mechanism of Tor regulation mediated by 14-3-3 interaction with Tctp and Rheb.
provenance
Made available in Cube on 2018-09-28T10:18:34Z (GMT). No. of bitstreams: 0
language
English
author
Le, Thao Phuong
Vuong, Linh Thuong
Kim, Ah-Ram
Hsu, Ya-Chieh
Choi, Kwang-Wook
orcid
Kim, Ah-Ram/0000-0001-9597-6759
accessioned
2018-09-28T10:18:34Z
available
2018-09-28T10:18:34Z
issued
2016
citation
NATURE COMMUNICATIONS(7)
issn
2041-1723
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/473403.do
Funder
교육부
Funding Program
BK21플러스사업(0.5)
Project ID
1345258171
Jurisdiction
Rep.of Korea
Project Name
BK21 PLUS BioKAIST Initiative
rights
openAccess
type
article


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