(Z)-5-(2,4-Dihydroxybenzylidene)thiazolidine-2,4-dione PreventsUVB-Induced Melanogenesis and Wrinkle Formation through SuppressingOxidative Stress in HRM-2 Hairless Mice

Collection with item attached
2016
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/473394.do
DOI
10.1155/2016/2761463
Title
(Z)-5-(2,4-Dihydroxybenzylidene)thiazolidine-2,4-dione PreventsUVB-Induced Melanogenesis and Wrinkle Formation through SuppressingOxidative Stress in HRM-2 Hairless Mice
Description
This work was supported by the National Research Foundation of Korea(NRF) grant funded by the Korean government (MSIP) (Grant no.2009-0083538) and by the Basic Science Research Program through theNational Research Foundation of Korea (NRF) funded by the Ministry ofEducation, Science and Technology (Grant no. NRF-2013R1A1A2009949). Theauthors thank Aging Tissue Bank for providing materials and researchinformation.
abstract
Background. Uncontrolled melanogenesis and wrinkle formation are an indication of photoaging. Our previous studies demonstrated that (Z)-5-(2,4-dihydroxybenzylidene)thiazolidine-2,4-dione (MHY498) inhibited tyrosinase activity and melanogenesis in vitro. Objective. To examine in vivo effects of MHY498 as an antiaging compound on UVB-induced melanogenesis and wrinkle formation, we topically applied MHY498 on dorsal skin of HRM-2 hairless mice. Methods. Using histological analysis, we evaluated effects of MHY498 on melanogenesis and wrinkle formation after UVB exposure. In addition, related molecular signaling pathways were examined using western blotting, fluorometric assay, and enzyme-linked immunosorbent assay. Results. MHY498 suppressed UVB-induced melanogenesis by inhibiting phosphorylation of CREB and translocation of MITF protein into the nucleus, which are key factors for tyrosinase expression. Consistently, tyrosinase protein levels were notably reduced in the dorsal skin of the hairless mice by MHY498 treatment. Furthermore, MHY498 inhibited UVB-induced wrinkle formation and collagen fiber destruction by increasing type 1 procollagen concentration and decreasing protein expression levels of MMPs, which play an essential role in collagen fiber degradation. As a mechanism, MHY498 notably ameliorated UVB-induced oxidative stress and NF-kappa B activation in the dermal skin of the hairless mice. Conclusion. Our study suggests that MHY498 can be used as a therapeutic or cosmetic agent for preventing uncontrolled melanogenesis and wrinkle formation.
provenance
Made available in Cube on 2018-09-28T10:18:17Z (GMT). No. of bitstreams: 0
language
English
author
Lee, Bonggi
Moon, Kyoung Mi
Kim, Seong Jin
Kim, So Hee
Kim, Dae Hyun
An, Hye Jin
Jeong, Ji Won
Kim, Ye Ra
Son, Sujin
Kim, Min Jo
Chung, Ki Wung
Lee, Eun Kyeong
Chun, Pusoon
Ha, Young Mi
Kim, Min-Sun
Mo, Sang Hyun
Moon, Hyung Ryong
Chung, Hae Young
accessioned
2018-09-28T10:18:17Z
available
2018-09-28T10:18:17Z
issued
2016
citation
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
issn
1942-0900
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/473394.do
Funder
교육부
Funding Program
BK21플러스사업(0.5)
Project ID
1345258417
Jurisdiction
Rep.of Korea
Project Name
Regulation of Resolution Information with Marine Products
rights
openAccess
type
article


Files in This Item

There are no attached files.